p53 mutations in HPV-negative vulvar squamous cell carcinomas – a preliminary study of 31 cases

Introduction: Less than 50% of vulvar SCC worldwide arise through transforming HPV-infections. The carcinogenesis of HPV-negative SCC is poorly understood, but chronic inflammation and p53 mutations have been implicated. We analyzed p53 mutations in 31 well documented HPV-negative vulvar SCCs from a previous study (Regauer et al, JAAD, 2014). Material and methods: DNA of formalin-fixed and Paraffin embedded tumor was extracted and the full coding sequence of the TP53 gene was analyzed using the Ion Torrent sequencing platform (Thermo Fisher) and the TP53 research Ampliseq panel. IRB approval was obtained (20.255…