Age-related carbonylation in glomeruli of diabetic mice influences anti-oxidative defense mechanisms

Background: In addition to the well-established role of protein carbonylation in oxidative stress, this oxidation process may also play a role in cell signal transduction. This study aims to investigate the influence of age-dependent protein carbonylation in the kidney of diabetic mice on the expression and activity of the cytosolic anti-oxidative selenoenzyme glutathione peroxidase (GPx). Methods: In addition to standard methods, dinitrophenylhydrazine (DNPH) derivatization has been used to detect carbonylated proteins in diabetic Ins2Akita mice. GPx activity was determined using a luminescent activity assay. R…