Isolation of circulating tumor cells using CellCelector post CellSearch enables characterization of single cells
M Neumann, S Schömer, Y Decker, H Schneck, M Fleisch, R Neves, N Stoecklein, T Fehm, F Meier-Stiegen, H Neubauer, D Niederacher · Senologie - Zeitschrift für Mammadiagnostik und -therapie · 2015 · Heft 05
Bibliografische Angaben
Zusammenfassung
Background: Circulating tumor cells (CTC) are rare cells and dissociated successfully from the primary tumor into the blood stream. Only a small proportion is capable to extravasate and form metastases. The presence of CTCs is associated with increased risk to develop metastases. Understanding the heterologous CTC biology enables the choice of the optimal therapy and improves the prognosis. Therefore, detection and isolation of CTCs is necessary. Here we show a workflow to characterize CTCs by combining the advantages of both the CellSearch®- and CellCelector™-System. Material and Methods: Single cells were clas…
