Epigenetic changes contribute to hepatic stellate cell activation

Introduction: Hepatic stellate cells (HSC) were recently identified as mesenchymal stem cells of the liver that exhibit a remarkable differentiation potential. The differentiation is preceded by an activation process. The aim of this study is to characterize the dynamics of DNA methylation during HSC activation to provide information about the epigenetic regulation of adult stem cells. Methods: Primary rat HSC were activated in cell culture within 7 days. DNA methylation of quiescent and activated HSC was determined using ELISA and immunofluorescence with 5mC-specific antibodies for global as well as reduced rep…