CareLit Fachartikel

Targeting mutant TP53 in ALL

S Demir, G Selivanova, E Tausch, L Wiesmüller, S Stilgenbauer, G te Kronnie, KM Debatin, LH Meyer · Klinische Pädiatrie · 2016 · Heft 05

Dokument
1146018
CareLit-ID
Jahr
2016
Publikation
PDF
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Metadaten
DOI
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Bibliografische Angaben

Zeitschrift
Klinische Pädiatrie
Autor:innen
S Demir, G Selivanova, E Tausch, L Wiesmüller, S Stilgenbauer, G te Kronnie, KM Debatin, LH Meyer
Ausgabe
Heft 05 / 2016
Seiten
–
Erschienen: 2016-05-02 00:00:00
ISSN
0300-8630
DOI
10.1055/s-0036-1582498

Zusammenfassung

Introduction: In ALL, TP53 mutations (TP53mut) are increased at relapse and associated with poor prognosis and resistance. APR-246 binds to mutant p53 and restoring wt conformation. Methods: TP53mut were analyzed by dHPLC and Sanger sequencing, apoptosis by AnnexinV/PI, p53 targets by western blotting, p53 silencing by lentiviral shRNA, and phosphorylation by phosphoflow cytometry. Results: APR-246 exposure led to apoptosis in TP53mut ALL and induced functional p53 re-activation indicated by p53Ser15 phosphorylation including expression of downstream targets in a p53 dependent manner in TP53mut but not TP53wt AL…

Schlagworte

Apoptosis Klinische Pädiatrie