CareLit Fachartikel

Utilization of hiPSC in leukemia research

AM Husa, MR Strobl, A Strajeriu, M Wieser, S Strehl, R Grillari, K Fortschegger · Klinische Pädiatrie · 2016 · Heft 05

Dokument
1146021
CareLit-ID
Jahr
2016
Publikation
PDF
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Metadaten
DOI
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Bibliografische Angaben

Zeitschrift
Klinische Pädiatrie
Autor:innen
AM Husa, MR Strobl, A Strajeriu, M Wieser, S Strehl, R Grillari, K Fortschegger
Ausgabe
Heft 05 / 2016
Seiten
–
Erschienen: 2016-05-02 00:00:00
ISSN
0300-8630
DOI
10.1055/s-0036-1582528

Zusammenfassung

Introduction: B-cell precursor acute lymphoblastic leukemia is the most common childhood cancer and is characterized by specific genetic lesions. To date, there are no human cellular model systems that recapitulate the disease and may reveal the impact of different mutations. Thus, we aim to use directed differentiation of human induced pluripotent stem cells (hiPSC) as a new model system. Method: To differentiate hiPSC into hematopoietic cells, we compared different methods: coculture with murine stromal cells, embryoid body formation, and monolayer cultures. Differentiation of hiPSC into CD34+ hemato-endotheli…

Schlagworte

Cells Hipsc Differentiation Human Model Different System Coculture Stromal Embryoid Leukemia Research Klinische Pädiatrie