Genome wide methylation analysis of gliomas by a microarray based technology

Changes of DNA methylation play a central role in carcinogenesis. Extensive hypermethylation and consecutive transcriptional silencing of tumorsuppressor genes has been documented in multiple tumor entities. The development of a microarray based technology known as differential methylation hybridization has significantly improved the genome wide search for hypermethylated genes. Based on this protocol we have developed a novel microarray containing 7680 CpG rich fragments of the human genome to investigate epigenetic alterations in cancer. Here we used this technology to identify novel genes de novo methylated i…