Analysis of expression patterns in the brains of tet-off promoter mice
J Boy, TB Leergaard, T Schmidt, C Holzmann, M Niwar, S Nuber, S Haas, S Prusiner, A Wree, JG Bjaalie, O Riess · Aktuelle Neurologie · 2004 · Heft 09
Bibliografische Angaben
Zusammenfassung
In traditional transgenic mouse models for neurological diseases, the transgene is constitutively expressed in invariable brain regions. The „Tet-Off-System“ developed by Dr. H. Bujard (ZMBH Heidelberg) however allows the generation of inducible transgenic mouse models and flexibility in the selection of the targeted brain regions. This system is based on two constructs: The promoter construct controls the expression of the so called tTA (Tetracycline transactivator) gene product. The binding of this protein to a Tetracycline responsive element (TRE) in the responder construct induces the transcription of the ge…
