CareLit Fachartikel
Cryopreservation of Precision Cut Tissue Slices (PCTS): Investigation of Morphology and Reagibility
HU Kasper, E Konze, N Kutinová Canová, U Drebber, HP Dienes · Zeitschrift für Gastroenterologie · 2005 · Heft 01
Dokument
1162791
CareLit-ID
Jahr
2005
Publikation
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Metadaten
DOI
ja
zitierfähig
Bibliografische Angaben
Zusammenfassung
Introduction: PCTS are used to study toxicity and metabolism of xenobiotics in vitro. We adapted this system for functional morphological research in liver tissue, e.g. for apoptosis investigation. To overcome the shortage of liver tissue, to optimize standardization and to get independence from the time of operation, a storage system of PCTS would be useful. Therefore, we investigated the possibility of cryopreservation of PCTS. Methods: PCTS were prepared from 4 porcine livers using Brendel Vitron Tissue Slicer. Four different freezing preparations were used. Half of the PCTS were exposed to ice cold RPMI medi…
Schlagworte
Cryopreservation
PCTS
reagibility
Apoptosis
After
Actinomycin D
Actinomycin
Zeitschrift für Gastroenterologie
