Effect of Ischemic Treatment of Human Liver Tissue on the Subsequent Isolation of Adult Liver Stem Cells

Isolation of adult liver stem cells is difficult because the cells share many morphologic and phenotypic features with other hepatic cells. Previous studies in our laboratory indicated that adult liver stem cells might be more resistant to ischemic stress than the differentiated liver cell populations. Therefore, it could be possible to enrich for liver stem cells through damaging the other liver cells by ischaemic treatment of the tissue. The aim of this study was to examine the effect of an ischemic treatment of human liver tissue on the subsequent isolation of adult liver stem cells. Human liver tissue pieces…