Degree of hepatocytes differentiation and expression levels of liver specific transcription factors: influence of Hepatitis B Virus replication
M Quasdorff, U Zedler, M Hoesel, F Bohne, A Untergasser, TH Nguyen, D Trono, T Goeser, U Protzer · Zeitschrift für Gastroenterologie · 2006 · Heft 01
Bibliografische Angaben
Zusammenfassung
Aims: Hepatitis B virus (HBV) replication is restricted to hepatocytes. But not all hepatocytes containing HBV genomes support viral replication. We studied the cellular differentiation state and expression levels of liver specific transcription factors essential for efficient HBV replication. Freshly isolated primary human hepatocytes (PHH) were compared with hepatoma cell lines HepG2 and HuH7 and the hepatocyte cell line pop10, replicating HBV at high, medium and low levels, respectively. Methods: We quantified expression of liver specific transcription factors (HNF4alpha, HNF1alpha/beta, HNF3alpha/beta/gamma,…
