RAGE modulates neuronal glyoxalase-1 transcription, expression and activity
SB Stoyanov, GM Haag, I Konrade, IK Lukic, P Humpert, P Thornalley, SR Thorpe, PP Nawroth, A Bierhaus · Diabetologie und Stoffwechsel · 2006 · Heft 06
Bibliografische Angaben
Zusammenfassung
Introduction: Methylglyoxal (MG) is a precursor of a variety of advanced glycation endproducts (AGEs), which are supposed to contribute to the development of late diabetic complications via interaction with their cellular receptor RAGE. MG and other AGE-precursors are detoxified by the glyoxalase-system. Aim: We study expression of the AGE-detoxifying enzyme glyoxalase-1 (GO-1) in peripheral nerves and dorsal root ganglia (DRG) of healthy and diabetic wildtype (WT)- and RAGE-/– mice to determine the interaction of RAGE and GO-1. Methods: GO1 transcription (Real time PCR), -expression (Western Blot and IHC) and -…
