CareLit Fachartikel
High sensitive pool screening of point mutations in BRCA–1 using chemiluminescence detection
S Albrecht, C Schönfels, T Zimmermann, W Distler · Geburtshilfe und Frauenheilkunde · 2008 · Heft 09
Dokument
1164725
CareLit-ID
Jahr
2008
Publikation
PDF
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Metadaten
DOI
ja
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Bibliografische Angaben
Zusammenfassung
A nonisotopic pool-screening method of point mutations (e.g. SNP) and small insertions / deletions in genetic samples is presented. Based on a formerly described (allele-specific pool-screening, the semi nested PCR is substituted by a one-step method in combination with solid-phase technique and chemiluminometric detection [3]. The allele-specific PCR was designed to discriminate between T and G changing the amino acid 300 from Cysteine to Glycine in the breast cancer gene 1 (BRCA–1). The PCR products were coupled via their Biotin label to Streptavidin-coated microtiter plates. For chemiluminometric measurement…
Schlagworte
BRCA-1
Breast Cancer
Chemiluminescence
Dioxetane
Light Reaction
PCR
Poolscreening
Allele
Population
Alleles
Digoxigenin
Cysteine
Biotin
Breast
Geburtshilfe und Frauenheilkunde
