The oncolytic adenovirus Adp53-sensor for selective replication in p53-altered tumors and potential target spectrum is not limited by the p73 status

Oncolytic virotherapy provides a promising mean for the treatment of solid tumors. Central goals of virotherapy are tight restriction of viral replication to tumor cells and a broad range of potential target tumors. Aim of our study was the establishment of a conditionally replicating adenovirus able to recognize the transcriptional p53-status of target cells and to consequently respond by the selective onset of viral replication in cells lacking functional p53. Therefore, we constructed Adp53-sensor expressed E1A under control of a GAL4-binding CMV-promoter and GAL4KRAB-repressor expression was controlled by th…