NeoHepatocytes generated from Patients with chronic Liver Disease can be used for autologous Cell Transplantation

Recently, we have shown that injection of hepatocyte-like cells can increase the survival in rats after extended liver resection. In order to apply this technology in humans with chronic liver diseases in an autologous setting, it is mandatory to verify whether Neohepatocyte-derived cells from these patients show similar metabolic patters as NeoHeps isolate from healthy controls. NeoHepatocytes were generated from monocytes of alcoholic patients and healthy controls and compared to human hepatocytes. We measured glucose and urea production as well as hepatocyte markers and the TGF-ß signaling pathway using PCR,…