Disseminated tumor cells in bone marrow of breast cancer patients–Comparison of immunocytochemistry and RT-PCR
T Fehm, S Becker, MJ Banys, G Becker-Pergola, S Duerr-Stoerzer, N Krawczyk, EF Solomayer · Geburtshilfe und Frauenheilkunde · 2006 · Heft 09
Bibliografische Angaben
Zusammenfassung
Background Since 1999 immunocytochemistry (ICC) has been regarded as the standard method of choice for detection of disseminated tumor cells (DTC) in bone marrow (BM). In the meantime molecular-based assays were developed, such as RT-PCR. The purpose of our investigation was to evaluate both techniques with respect to their sensitivities on the basis of 417 bone marrow aspirates from breast cancer patients. Methods 417 bone marrow aspirates from primary breast cancer patients were processed with both methods. Immunocytochemistry After Ficoll enrichment of 10ml bone marrow, cytospins were prepared and stained usi…
