Mesenchymal stromal cells: 1. in vitro expansion with platelet lysate, 2. in vivo administration for acute renal failure: Recovery through paracrine mechanism.

Human bone marrow mesenchymal stromal cells (hMSC) are currently investigated for a variety of therapeutic applications. However, most expansion protocols still use fetal calf serum (FCS) as growth factor supplement which is a potential source of undesired xenogeneic pathogens. We established an expansion protocol for hMSC based on GMP-produced medium supplemented with platelet lysate (PL) obtained from human thrombocyte concentrates. Compared to FCS-supplemented culture conditions we found a significant increase of both CFU-F as well as cumulative cell numbers after expansion. Cells expanded by this protocol me…