Aktivierte neutrophile Granulozyten induzieren DNA Methylierung in Darmepithelzellen durch Regulierung der DNA Methyltransferasen DNMT3A und DNMT3B

Background/Aim: Ulcerative colitis is a disease with a high risk for the development of colorectal cancer. We have previously shown that activated neutrophils (PMN) induce a MSH2-dependent G2/M arrest and replication errors in colon epithelial cells (Campregher et al., Gut 2008). Utilizing the same co-culture system we intended to investigate possible epigenetic changes in colon epithelial cells induced by activated PMNs. Methods: Freshly isolated PMNs were activated with phorbol myristate acetate, and superoxide (O2-) release was measured by lucigenin-amplified chemiluminescence. HT29 colon epithelial cells (ta…