CareLit Fachartikel
Expression profile and functional activity of Platelet-derived growth factor-C and -D in bile duct-ligated rat livers and transdifferentiating hepatic stellate cells
E Borkham-Kamphorst, AM Gressner, R Weiskirchen · Zeitschrift für Gastroenterologie · 2007 · Heft 01
Dokument
1166890
CareLit-ID
Jahr
2007
Publikation
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Metadaten
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Bibliografische Angaben
Zusammenfassung
Background/Aims: We analyzed the expression of the novel identified platelet-derived growth factor-C and -D (PDGF-C, PDGF-D) in an experimental bile duct-ligated rat model and compared them to those of PDGF-A, PDGF-B and their receptors, and assessed the biological function of these novel cytokines in cultured hepatic stellate cells (HSC). Methods: The mRNA for PDGF-A, -B, -C, and –D, as well as for PDGF receptor-α and -β chains (PDGFRα and PDGFRβ) in normal and fibrotic rat livers, was assessed quantitatively by real time PCR. The protein level of PDGF-D was quantified by immunoblotting and immunohistochemistry…
Schlagworte
PDGF
bile-duct ligation
cellular activation
hepatic stellate cells
liver fibrosis
myofibroblast
transdifferentiation
Cytokines
Cytokine
Zeitschrift für Gastroenterologie
