Characterization of acid/base transporters in parietal cells isolated from human gastric biopsy samples
I Ignáth, Z Rakonczay Jr, T Takács, A Schnúr, V Venglovecz, A Szepes, Z Szepes, L Czakó, A Tiszai, A Rosztóczy, T Molnár, F Izbéki, J Lonovics, A Varró, P Hegyi · Zeitschrift für Gastroenterologie · 2007 · Heft 05
Bibliografische Angaben
Zusammenfassung
Background: The acid/base transporters of human parietal cells have not been characterized yet. However, these transporters may play a role in the mechanism of acid secretion. The aim of this study was to investigate the transporters of the parietal cells obtained from human biopsy samples and dissects the mechanisms relevant to human pathology. Methods: Gastric glands were isolated from human biopsy samples by enzymatic digestion and then cultured for 48h. Parietal cells were identified using FITC-conjugated Dolichos biflorus lectin. The intracellular pH (pHi) of parietal cells was measured by using the fluores…
