The neuroprotective compounds riluzole, minocycline, and memantine interact with AMPA-type glutamate receptors: a patch clamp study

Antagonizing glutamatergic neurotransmission by blockade of AMPA-type glutamate receptors is a promising pharmacological strategy in the treatment of chronic neurodegenerative diseases. We performed the patch-clamp technique in combination with an ultrafast solution exchange system to investigate the interaction of riluzole, minocycline and memantine with recombinant AMPA-type glutamate receptor channels. Homooligomeric GluR2flipGQ or non-desensitizing GluR2 L504Y channels were expressed in HEK293 cells. We conducted experiments following coapplication and preincubation protocols for the respective test compound…