CareLit Fachartikel
ER-stress activation in human hepatocellular cancer cells: an alternative death pathway induced by the pan-deacetylase inhibitor panobinostat
P Di Fazio, R Montalbano, K Quint, S Gahr, T Wissniowski, M Ocker · Zeitschrift für Gastroenterologie · 2010 · Heft 01
Dokument
1168051
CareLit-ID
Jahr
2010
Publikation
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Zusammenfassung
Background: We previously showed that panobinostat, a novel pan-deacetylase inhibitor, has a potent apoptotic activity in vitro and induces growth delay of HCC xenografts in nude mice. Panobinostat is able to induce cell death in HepG2 (p53wt) and in Hep3B (p53null) cell lines that is not dependent on canonical apoptotic pathways. Here we analyse the effect on ER-stress by panobinostat treatment. Methods: HepG2 and Hep3B cells were cultured under standard conditions and treated for 48h with 0.1µM panobinostat. Sub-G1 events were quantified by flow cytometry after propidium iodide staining and verified by immunof…
Schlagworte
ER stress
HDAC
apoptosis
liver cancer
After
Caspase 12
Acetylation
Zeitschrift für Gastroenterologie
