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Comparative analysis of Phase I and II Enzyme Activities in 6 Hepatoma Cell Lines Identifies Huh-7 Cells with the highest Potential to study Drug Metabolism

S Ehnert, J Lin, N Nussler, L Liu, AK Nussler · Zeitschrift für Gastroenterologie · 2010 · Heft 01

Dokument
1168053
CareLit-ID
Jahr
2010
Publikation
PDF
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Metadaten
DOI
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Bibliografische Angaben

Zeitschrift
Zeitschrift für Gastroenterologie
Autor:innen
S Ehnert, J Lin, N Nussler, L Liu, AK Nussler
Ausgabe
Heft 01 / 2010
Seiten
–
Erschienen: 2010-01-12 00:00:00
ISSN
0044-2771
DOI
10.1055/s-0029-1246399

Zusammenfassung

Gold standard to perform human drug metabolism studies is primary human hepatocytes. Their availability is limited by donor organ scarcity. Hepatoma cell lines are widely used as an alternative. Although, phase I and II drug metabolizing activities of these cell lines are substantially lower than in primary hepatocytes, their major advantage is immediate availability, standard culture conditions and unlimited life-span. Thus, aim of this study was to investigate the drug-metabolizing profile of 6 standard hepatoma cell lines (HepG2, Hep3B, Hep1.6, HCC-T, HCC-M and Huh-7) over a culture period of 10 passages. At…

Schlagworte

Hepatoma cell lines cytochrome P450 drug metabolism primary human hepatocytes Observation Cytochrome Zeitschrift für Gastroenterologie