Epigenetic based enrichment of cancer stem cells (CSC) in liver cancer: Mechanistic and clinical implications
JU Marquardt, C Raggi, JB Andersen, D Seo, I Avital, A Holczbauer, VM Factor, SS Thorgeirsson · Zeitschrift für Gastroenterologie · 2010 · Heft 01
Bibliografische Angaben
Zusammenfassung
Epigenetic mechanisms determine to a large extent fate and specific characteristics of cells and play a critical role in both stem cell and cancer biology. Epigenetic modulation directs stem cells towards pluripotency while concomitantly promoting differentiation of more mature derivatives. If the same epigenetic mechanisms are applicable to the CSC model, then epigenetic modulation might enrich the CSC population thus facilitating CSC isolation and rigorous evaluation. Five hepatoma cell lines were treated with Zebularine (ZEB), an effective DNMT1-inhibitor. Putative CSC were isolated using the “side population…
