Intravenous Application of Fluorescein for Confocal Laser Scanning Microscopy: Evaluation of Contrast Dynamics and Image Quality with Increasing Injection-to-Imaging Time

Background: Confocal laser scanning microscopy (CLSM) is reported to be a promising tool for in-vivo histopathology during endoscopy. The most commonly used fluorophore is fluorescein sodium applied intravenously (iv). However, so far, there are no objective data on contrast dynamics and image quality over time after injection of the substance. We therefore aimed to investigate the ideal time period for best CLSM imaging using this substance. Material and Methods: The proximal stomach of 4 anaesthetized pigs was examined via standard endoscopes with a portable confocal miniprobe. After injection of 1% fluorescei…