Protein kinase D2-regulated protein transport at the trans-Golgi network requires its targeting to this compartment by ADP-ribosylation factor 1

Introduction: The PKD family of serine/threonine kinases comprises PKD1/PKCµ, PKD2 and PKD3/PKCv. These kinases are involved in various fundamental biological processes including regulation of Golgi structure and function and consequently protein transport from the (trans-Golgi network) TGN to the plasma membrane. Whereas there is substantial data on the function of PKDs at the TGN, the recruitment of these kinases to a specific diacylglycerol pool at the TGN is less understood. The small GTPases of the ADP-ribosylation factor (ARF) family are also master regulators of the structure and function of the Golgi com…