Darbepoetin inhibits proliferation of Huh-7 and Hep3B cells via up-regulation of the tumor-suppressor gene p53
L Schyschka, J Neumann, R Mühl-Benninghaus, M Unger, U Stöckle, S Ehnert, A Nüssler · Zeitschrift für Gastroenterologie · 2011 · Heft 01
Bibliografische Angaben
Zusammenfassung
In 2002 Aranesp (Darbepoetin) was licensed for patients with solid tumors suffering from chemotherapy-dependent anemia. As a better blood supply may favor tumor growth and spreading, we wanted to investigate whether there are direct effects of Darbepoetin on liver tumor cell lines or primary human hepatocytes. Human hepatoma cell-lines used were HepG2, Hep3B, SkHep1, Huh-7, AKN1, HCC-T and HCC-M. Primary human hepatocytes were isolated by collagenase perfusion according to the ethical guidelines of the MRI. Cells were stimulated with different concentrations of Darbepoetin (0, 0.15, 0.31, 0.63, 1.26, 2.5, 5, 10,…
