Regulatory modules of miRNA and transcription factors affect gene expression in cultivated hepatocytes

Introduction: Gene expression pattern of isolated mouse hepatocytes differ from the in vivo situation. The following study was performed to identify transcription factors (TF) and miRNAs that modulate the gene expression in cultivated hepatocytes. Material and Methods: Primary mouse hepatocytes were cultivated for 48h on collagen coated monolayers and gene expression changes were determined at 24, 27, 30, 36 and 48h after seeding using Affymetrix gene arrays. The promoter regions of the differentially expressed genes were analyzed for over-represented transcription factor binding sites (TFBS) using EXPANDER (EXP…