Antiproliferative and antiviral effects of mutated IFN-alpha with increased IFN receptor binding affinity
MF Sprinzl, L Bührer, D Strand, G Schreiber, PR Galle, J Piehler, M Schuchmann · Zeitschrift für Gastroenterologie · 2011 · Heft 01
Bibliografische Angaben
Zusammenfassung
Background: IFN receptors located on the cellular membrane transduce IFN dependent signals and activate antiviral and antiproliferative effects. In this project we characterized the antiviral and antiproliferative action of mutated IFN-alpha (IFN-HEQ), which shows an increased affinity to IFNR1 compared to wild type IFN-alpha. Methods: Subgenomic HCV replicons (Huh7-HCVluc), which contain a luciferase reporter, were incubated with IFN-alpha, IFN-beta and IFN-HEQ at different concentrations. HCV replication was quantified by standard photometric analysis. To assess antiproliferative effects a MTT based assay was…
