The effect of endothelin-1 on human basophil function in vitro

Endothelin-1 (ET-1) has proinflammatory properties and contributes to allergic late-phase responses. As basophils play a key role in allergic rhinitis or asthma, we investigated the effect of ET-1 on basophils. Cells were isolated from venous blood of healthy donors via magnetic cell sorting (MACS®). Basophils migrated towards ET-1 [10–6-10–16 M] or MCP-1 [10–8 M] (positive control) for 90min in modified Boyden chambers. To explore whether ETA or ETB receptors are involved, cells were preincubated with BQ-123 [10–6-10–16 M] or BQ-788 [10–6-10–16 M]. Migration depth was quantified microscopically. For evaluating…