Molecular characterization of a new anti-idiotype antibody for active immunotherapy in neuroblastoma

Introduction: Glycolipid Disialoganglioside GD2 is a highly expressed but poorly immunogenic antigen in neuroblastoma (NB). An anti-idiotype antibody (AIT-ab), which mimics the paratope of GD2, could induce an active immune response against NB. Methods/Results: We developed a new AIT-ab called Ganglidiomab by immunizing BALB/c mice with murine ab 14G2A. Hybridomas were generated and clones secreting AIT-ab were identified. Further, total RNA from hybridoma cells was isolated and cDNA was synthesized. DNA fragments encoding for the variable regions of the heavy (VH) and light chain (VL) were amplified, followed b…