GATA1s exerts fetal stage-specific oncogenic effects in hematopoietic stem and progenitor cells

Introduction: Mutations of the hematopoietic transcription factor GATA1, leading to expression of a shorter N-terminal truncated protein (GATA1s) are associated with transient leukemia (TL) and acute megakaryoblastic leukemia (AMKL) in children with Down syndrome. However, the consequences of GATA1s expression in synergy with fetal background in human hematopoietic stem and progenitor cells (HSPCs) remain unknown. Methods: Colony-forming capacity and multilineage differentiation of GATA1/GATA1s transduced human CD34+ HSPCs from fetal liver (FL), cord blood (CB) and adult peripheral blood (G-CSF-mobilized) were i…