Increased CYP24A1 mRNA expression after 1,25-Dihydroxyvitamin D3 treatment of hepatocellular carcinoma cell lines in vitro and also in human liver cancer
E Horvath, P Lakatos, B Balla, PJ Kósa, I Kovalszky, V Pesti, D Németh, J Hasan, A Korompay, Á Somorácz, Z Schaff, P Kupcsulik, F Szalay · Zeitschrift für Gastroenterologie · 2011 · Heft 05
Bibliografische Angaben
Zusammenfassung
Background and aims: The active metabolite of vitamin D3, 1,25-dihydroxyvitamin D3 (1,25D3) inhibits cell growth in numerous tumors as colon, prostate, breast. The role of 1,25-D3 in the development of the hepatocellular carcinoma (HCC) has not yet been established. We aimed to study the mRNA expression of vitamin D inactivating CYP24A1 enzyme in HCC cell lines after 1,25D3 treatment in vitro and also in human HCC tissue samples. Methods: HepG2 and HUHneo (Univ. Heidelberg) cells were treated with 4 nM of 1,25D3 in Optimem medium and were incubated in two parallels for duration of 30, 60, 120 minutes, 5, 8, 10,…
