Cytoplasmic Aconitase – Isocitrate Dehydrogenase as Source of NADPH for Fatty Acid Synthesis in Prostate Cancer

Introduction: Prostate is a unique organ that produces and releases large amounts of citrate. This changes in prostate cancer where the level of citrate drops dramatically. As shown previously, prostate cancer cells can uptake extracellular citrate and use it for fatty acid (FA) sythesis. Here we tested the hypothesis that cytosolic ACNT (cACNT) and isocitrate dehydrogenase (cICD) are important for supplying the NADPH for FA synthesis. Materials and Methods: Normal PNT2-Ce and strongly metastatic PC-3M cells were use for comparative studies. Enzyme activities and substrate levels were measured using spectrophoto…