Identification of new targets of cisplatin resistance in ovarian cancer patients using combined transcriptome and methylation analyses

The standard therapy for Ovarian cancer consists of aggressive cytoreductive surgery followed by platinum-based chemotherapy. However, intrinsic or acquired platinum resistance in the majority of patients leads to high mortality rate. To gain new insights in resistance mechanisms and modified pathways, we performed combined whole-genome expression and methylation studies using Illumina BeadArrays. Eleven cryopreserved tissue samples from each platinum resistant and platinum sensitive ovarian carcinomas were selected. From consecutive tissue sections RNA and DNA was extracted and analysed for differences in gene…