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Molecular characterization of lincRNAs in Down syndrome associated leukemia

A Streltsov, S Emmrich, F Engeland, JH Klusmann · Klinische Pädiatrie · 2012 · Heft 04

Dokument
1171269
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Jahr
2012
Publikation
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Bibliografische Angaben

Zeitschrift
Klinische Pädiatrie
Autor:innen
A Streltsov, S Emmrich, F Engeland, JH Klusmann
Ausgabe
Heft 04 / 2012
Seiten
–
Erschienen: 2012-04-27 00:00:00
ISSN
0300-8630
DOI
10.1055/s-0032-1310469

Zusammenfassung

We analyzed the long intervening non-coding RNA (lincRNA) host genes (MIR100HG, LINC00478 and LINC00085) of the mir-99˜125 clusters. Those miRNA clusters reflect the phylogenetically most ancient miRNA clusters with important roles in hematopoietic stem and progenitor cell (HSPC) homeostasis and the development of acute megakaryoblastic leukemia (AMKL). By qRT-PCR-profiling, we could show that MIR100HG, LINC00478 and LINC00085 are overexpressed in AMKL cell lines (Meg-01, CMK, CMY) in contrast to other leukemic cell lines (K562, THP-1, Kasumi). For gain and loss of function studies of lincRNAs, we designed and e…

Schlagworte

Transplantation Klinische Pädiatrie