Characterization of oncogenes on chromosome 21 identified by shRNA-based viability screening

Introduction: Children with trisomy 21 (Down syndrome, DS) are predisposed to develop acute megakaryoblastic leukemia (DS-AMKL). Hypothesizing that the presence of an extra copy of human chromosome 21 (hsa21) is one basal requirement for the onset of DS-AMKL, we previously conducted a shRNA-based viability screening to identify those yet unknown oncogenic candidates. Methods: We analyzed how candidate oncogenes from the viability screening affect proliferation, self-renewal, apoptosis, colony-forming capacity and differentiation in vitro. Results: Knockdown of USP25 selectively mediated proliferation arrest and…