In vivo RNAi screening identified MKK4 as a new therapeutic target for the improvement of liver regeneration
T Wuestefeld, M Pesic, T Longerich, TW Kang, T Yevsa, D Dauch, A Potapova, R Rudalska, I Rittelmeyer, M Jarek, R Geffers, M Scharfe, F Klawonn, NP Malek, P Schirmacher, M Ott, A Vogel, MP Manns, L Zender · Zeitschrift für Gastroenterologie · 2013 · Heft 01
Bibliografische Angaben
Zusammenfassung
We established a unique system for performing direct in vivo RNAi screens to genetically dissect cellular signaling networks that regulate proliferation control of hepatocytes during liver damage and regeneration. By combining the well characterized FAH-/- mouse model with a transposon based vector system, we generated chimeric mouse livers. In these mouse livers each hepatocyte expresses FAH, a marker gene and a microRNA based shRNA. To such mouse livers any form of liver damaging protocol can be applied and the regenerative process can be studied. We conducted a first in vivo RNAi screen, using a focused shRNA…
