Continuous lipid treatment of primary human hepatocytes leads to an elevation of oxidative stress

Non-alcoholic fatty liver disease (NAFLD) is a growing disease in Western societies. It leads to durable morphologic and biochemical changes of liver tissue like inflammatory processes. Thereby a development of steatohepatitis, fibrosis and cirrhosis is possible. These cause decreased liver regeneration as well as less detoxification capacity and may lead to all degree of hepatic failure. Our aim was to establish a long term steatosis model based on primary human hepatocytes (PHH) in order to investigate steatohepatitis related inflammatory processes. PHH were isolated from resected human liver using a two step…