Peroxide-induced effects on spontaneous contraction of murine small intestine: Lack of inhibition by antioxidants

An experimental model of inflammatory reaction in murine small intestine was developed by characterizing the effects of oxidative stress on spontaneous contractions in vitro. The tissue specimen were obtained from wild type mice (C57Bl6) and mounted in an organ bath allowing the registration of contraction forces under isometric conditions (Merkel, Thesis, 2010). Rhythmic spontaneous contractions were observed under normal perfusion conditions with a frequency (F) of approx. 20 ± 3/min and a mean amplitude (A) of 1.8 ± 0.4 mN. In order to induce oxidative stress, hydrogen peroxide was applied (0.05 – 0.6 mM/l) a…