Molecular stratification of medulloblastoma: Comparison of histological and genetical methods to detect Wnt activated tumors

In a cohort of 188 pediatric medulloblastomas our aim was to identify the optimal methods in standard clinical practice to detect those with Wnt activation, which is associated with good outcome. Therefore, nuclear accumulation of ß-Catenin was analyzed by immunohistochemistry (IHC) using 3 different automated staining systems. Also SSCP (single-strand conformation polymorphism) analysis and direct Sanger sequencing of CTNNB1 exon 3 was done. Finally, copy number aberrations of chromosome 6 were analyzed by MLPA (multiplex ligation-dependent probe amplification) and by molecular inversion profiling. 22 samples s…