Analysing the Mutational Status of PIK3CA in Circulating Tumor Cells from Metastatic Breast Cancer Patients
H Schneck, C Blassl, F Meier-Stiegen, T Fehm, H Neubauer · Senologie - Zeitschrift für Mammadiagnostik und -therapie · 2013 · Heft 06
Bibliografische Angaben
Zusammenfassung
The frequently altered phosphatidylinositol-3-kinase (PI3K)/Akt signaling pathway is involved in the regulation of cellular processes required for carcinogenesis. The aim of the project was to develop a method to identify hotspot mutations in the PIK3CA gene locus in circulating tumor cells (CTC) of metastatic breast cancer (metBC) patients. From 65 enrolled CTC-positive metBC patients a total number of 81 peripheral blood samples were analysed by CellSearch®. Genomic DNA of enriched CTCs was isolated, amplified and analyzed for PIK3CA mutations in exons 9 and 20 leading to either E542K, E545K or H1047R amino ac…
