Role of methylthioadenosine phosphorylase (MTAP) in hepatic stellate cell activation
B Czech, D Valletta, K Dettmer, M Müller, A Bosserhoff, P Oefner, C Hellerbrand · Zeitschrift für Gastroenterologie · 2013 · Heft 08
Bibliografische Angaben
Zusammenfassung
The activation of hepatic stellate cells (HSCs) is a key event of hepatic fibrosis, and the polyamine metabolic pathway has been implicated to affects hepatic fibrosis. Methylthioadenosine phosphorylase (MTAP) the rate-limiting enzyme in the methionine and adenine salvage pathway catalyzes the phosphorylation of 5'-deoxy-5′-(methylthio)adenosine (MTA), which is a by-product of polyamine synthesis. The aim of this study was to assess the role of MTAP in HSCs. Methods and Results: Quantitative PCR and Westernblot analyses revealed increased MTAP expression during in vitro HSC activation, and immunohistochemical an…
