Preliminary study of the ultrasound/fluorescent bi-functional contrast agent for rabbit's normal lymph node imaging

Purpose: To prepare a lymph node targeted ultrasound/fluorescence bi-functional imaging contrast agents, and observe the effects both on contrast-enhanced ultrasound (CEUS) and vivo near infrared fluorescence (NIR) imaging through animal experiments. Material and methods: Assembling chimeric lymph node targeted ligand (phosphatidylserine) and near-infrared fluorescent substance to form bi-functional contrast microbubbles. The morphology and size distribution were detected by optical microscope and Malvern potential tests. Five normal New Zealand white rabbits were subcutaneous injected with contrast agent in bil…