An algorithm that predicts the viability and the yield of isolated human hepatocytes from surgical remnant tissues

Introduction: The liver is an organ that carries out a wide range of functions, such as detoxification, metabolism and homeostasis. As such, hepatocytes are an important model for a large variety of research questions. In particular, the use of hepatocytes is especially important in the fields of pharmacokinetics, toxicology and translational research. In order to obtain hepatocytes suitable for use as a model, hepatocytes with high viability and yield must be isolated. Therefore, this study aims to identify donor characteristics, intra-operative factors, tissue processing and cell isolation parameters that affe…