Development of an orthotopic HCC mouse model for analysis of tumor-specific CD8 T cell responses

Immunotherapy against liver cancer is a promising approach that needs to be further developed for the CD8 T cells. We have established an orthotopic HCC mouse model in order to study the influence of hepatocellular carcinoma on cytotoxic CD8 T cells. Hepatic tumors were generated in vivo using the "Sleeping beauty" transposon system. The transfection of murine hepatocytes with the plasmids that contain cDNA coding for oncogenes was performed by hydrodynamic injection. Tumor development in the mice livers began after the SB Transposase integrated the oncogenes into the hepatocyte genome. We have used the oncogeni…