Prediabetic phenotype in transgenic pigs expressing the mutant insulin C93S
C Braun-Reichhart, E Streckel, N Klymiuk, N Herbach, C Landbrecht-Schessl, A Wünsch, B Kessler, M Kurome, S Krebs, H Nagashima, H Blum, R Wanke, B Aigner, E Wolf, S Renner · Diabetologie und Stoffwechsel · 2014 · Heft 05
Bibliografische Angaben
Zusammenfassung
Aims: Mutant INS gene-induced diabetes of youth (MIDY) in humans is caused by missense mutations of the insulin gene. The severity of the disease depends on the type of mutation. We generated transgenic pigs expressing INS C93S to complement the previously established INSC 94 Y transgenic pig model, which develops permanent neonatal diabetes mellitus (Renner et al., Diabetes 62, 1505 – 11, 2013). Methods: The expression vector included porcine INS regulatory sequences and the INS C93S coding sequence. Pools of stable transfected cell clones were used for somatic cell nuclear transfer. The ratio of INSC 93 S to w…
