Microglial polarization is independent of glucose levels and methylglyoxal

Microglia perform various functions, either inflammatory response or protection, depending on their current microenvironment. Diabetic Retinopathy is driven through local inflammations mediated by microglial cells. The question arises, whether high glucose levels or methylglyoxal are able to induce microglial polarization on their own. Primary murine microglial BV2 cells were used for the stimulation, which was performed with DMEM containing different concentrations of glucose and methylglyoxal for 72 hours (5mmol/L; 25mmol/L; 50mmol/L; 100mmol/L of glucose or 500µmol/L; 5mmol/L; 10mmol/L of methylglyoxal). Afte…