TLR-activated monocytes express IFN-lambda receptor 1 and trigger IFN-gamma secretion of NK cells after stimulation with IFN-lambda
B Krämer, BS Sastre Turrión, A Glässner, F Wolter, P Kokordelis, M Eisenhardt, HD Nischalke, CP Strassburg, U Spengler, J Nattermann · Zeitschrift für Gastroenterologie · 2014 · Heft 08
Bibliografische Angaben
Zusammenfassung
Background: Immungenetic data suggest that lambda-IFNs (l-IFN) may modulate anti-HCV activity of natural killer (NK) cells. However, NK cells do not express the IFN-lambda receptor 1 (IFN-lR1). Thus, we studied the hypothesis that l-IFNs act on other leucocyte subsets, which then indirectly modulate NK cell activity. Methods: IFN-lR1 expression was studied by flowcytometry on circulating leukocyte subsets in healthy controls (n = 10). Peripheral blood monocytes were isolated using MACS technology and stimulated with LPS, TLR7 agonist R848 and HCV core, respectively. Then IFN-lR1 expression was determined by flow…
