Analysis and applicability of different in vitro models of Glioblastoma multiforme

Glioblastoma multiforme (GBM) still has a very dismal prognosis. Numerous efforts are made to investigate cellular mechanisms in GBM tumor cells and to develop new, more effective personalized therapies. For the development of novel treatment strategies optimal in vitro models of GBMs are essential, generated from individual patients and analysed at early passages to avoid culture artefacts. Up to date a variety of cell culture methods with different properties are available. However, differences between the classical serum containing cell culture and the so called cancer stem cell culture (serum free, supplemen…